ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 33 kDa band observed in humanthymus lysates (calculated MW of 33.2 kDa according to NP_002459.1). Recommended concentration: 0.03-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human tonsil shows staining of clusters of cells some of which are likely plasma cells. Recommended concentration, 4-6μg/ml
MYD88 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MYD88 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.