ELISA: Antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approximately 75 kDa and 150 kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/mL antibody staining. The150 kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6 kDa according to NP_055606.1. Recommended concentration: 0.1-0.3 μg/mL.
MELK antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MELK antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.