ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 45 kDa band observed in human brain lysates, kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1 kDa according to NP_002737.1). Recommended concentration: 0.1-0.3 μg/mL.
MAPK3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MAPK3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.