ELISA: Antibody detection limit dilution >1:128,000. Western Blot: Approximately 32 kDa band observed in mouse heart lysates (predicted MW of 31 kDa according to NP_076173.1). Recommended for use at 0.5-2 μg/mL.
LIME1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LIME1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.