ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 57 kDa band observed in A549 lysate . In transfected HEK293 transiently expressing IRF5 a strong band of approximately 60 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8 kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3 μg/mL.
IRF5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
IRF5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.