ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 100 kDa band observed in NIH/3T3 lysate (calculated MW of 92.5 kDa according to NP_003290.1). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Recommended concentration, 3-5 μg/mL.
HSP90B1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HSP90B1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Mouse, Rat