ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 18 kDa band observed in human liver lysates (calculated MW of 16.4 kDa according to NP_002090.2). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows bright staining of erythrocytes. Recommended concentration, 2-4 μg/mL.
Glycophorin A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GlycopH orin A antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.