ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 22 kDa band observed in human liver and kidney lysates (calculated MW of 22.1 kDa according to NP_000572.2). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human cerebral cortex shows staining of microglia. Recommended concentration, 3-5 μg/mL.
Glutathione Peroxidase 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Glutathione peroxidase 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.