ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 36 kDa band observed in human liver, testis and tonsil lysates and in mouse spleen lysates (calculated MW of 36.1 kDa according to NP_002037.2). Recommended for use at 0.2-1 μg/ml.
GAPDH antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GAPDH antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.