ELISA: Antibody detection limit dilution 1:64000. Western Blot: A 55-60 kDa band observed in human liver lysate (calculated MW of 58.9 kDa according to NP_006648. Recommended concentration: 0.03-0.5 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5 μg/mL.
FTCD antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FTCD antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.