ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 48 kDa band observed in human cerebellum, lung and testis lysates (calculated MW of 47.1 kDa according to NP_004466.2). Recommended concentration: 0.1-0.3 μg/mL.
Flotillin 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Flotillin 2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.