ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 80 kDa band observed in mouse and rat brain lysates (calculated MW of 61.6 kDa according to human NP_005222.2 and of 61.2 kDa according to mouse NP_031829.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paradis et al, J Cell Sci. 2008 Jun 15;121 (Pt 12) :1965-72; PMID 18495841). Recommended concentration: 0.01-0.03 μg/mL.
Cortactin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cortactin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.