ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 18 kDa band observed in mouse Tesits and mouse kidney lysates (calculated MW of 15.5 kDa according to NP_034106.2). Recommended concentration: 0.01-0.03 μg/mL.
Cystatin C antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cystatin C antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.