ELISA: Antibody detection limit dilution >1:128,000. Western Blot: Approximately 23 kDa band observed in human liver lysate (calculated MW of 25.7 kDa according to NP_652763). Recommended concentration: 1-3 μg/ml.
Claudin 14 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Claudin 14 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.