ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 70 kDa band observed in human placenta lysates (calculated MW of 70.5 kDa according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3 μg/mL.
ChAT antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ChAT antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.