ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 75-80 kDa band observed in mouse and rat brain lysates (calculated MW of 72.3 kDa according to NP_034021.1). Recommended concentration: 0.2-0.5 μg/mL.
ChAT antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ChAT antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.