ELISA: Antibody detection limit dilution 1:32,000. Approximately 26 kDa band observed in human breast and prostate lysates (calculated MW of 22.1 kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27;102 (39) :14028-33; PMID: 16169901). Recommended concentration: 1-3 μg/mL.
CDKN1B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CDKN1B antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.