ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 20 kDa band observed in lysates of A431, HeLa and HepG2. (calculated MW of 20.8 kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 μg/mL. Immunohistochemistry: In paraffin embedded human placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3 μg/mL.
CBX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CBX3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse