ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 21 kDa and 25 kDa observed in human heart, lung and spleen lysates. These bands correspond to earlier findings in literature with different antibodies (Wiechen et al Am J Pathol. 2001 Mar;158 (3) :833-9, PMID: 11238032 ). In transfected HEK293 transiently expressing Caveolin 1 a band of approximately 23 kDa is observed. This band is not observed in the non-transfected HEK293. This protein has a calculated MW of 20.5 kDa according to NP_001744.2. Recommended concentration: 0.3-1 μg/mL.
Caveolin-1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Caveolin-1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.