ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in human kidney and liver lysates (calculated MW of 59.8 kDa according to NP_001743.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: Immunohistochemistry: In paraffin embedded human liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4 μg/mL.
Catalase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Catalase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat