ELISA: Antibody detection limit dilution 1:32000 Western Blot: Approximately 70 kDa band seen in Daudi lysate. Recommended for use at 3-5 μg/mL. Please note that although the predicted MW of BLNK is Approximatelyimately 50 kDa, it runs at 70-75 kDa on SDS-PAGE.
BLNK antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
BLNK antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.