ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 45 kDa band seen in Jurkat and HeLa cell lysates. Recommended for use at 0.5-2 μg/mL.
Aurora Kinase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Aurora kinase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.