ELISA: Antibody detection limit dilution 1:16,000. Western Blot: A 50-55 kDa band observed in human hepatoblastoma HepG2 lysates (calculated MW of 55.8 kDa according to NP_005456 and 54.0 kDa according to NP_859029). Recommended concentration: 1-3 μg
AKT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
AKT3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat