ELISA: Antibody detection limit dilution 1000. Western Blot: Approx 55 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1 kDa according to Human NP_032254.1 and Mouse NP_032254.1). Recommended concentration: 0.3-1 μg/mL.
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Hdac1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.