ELISA: Antibody detection limit dilution 64000. Western Blot: Approx 40 kDa band observed in nuclear, but not in cytosolic HeLa lysate (calculated MW of 37.7 kDa according to NP_003185.1). Recommended concentration: 0.3-1 μg/mL.
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TBP antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.