ELISA: Antibody detection limit dilution 32000. Western Blot: Approx 70 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9 kDa according to NP_001070.2). Recommended concentration: 0.3-1 μg/mL.
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ZAP70 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.