ELISA: Antibody detection limit dilution 8000. Western Blot: Preliminary experiments gave an approx 70-75 kDa band in Human Kidney and Liver lysates after 0.2 μg/mL antibody staining.
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TRIM6 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.