ELISA: Antibody detection limit dilution 128000. Western Blot: Preliminary experiments gave an approx 200 kDa band in lysates of cell lines Jurkat and Molt4 after 0.05 μg/mL antibody staining.
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HMHA1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.