Tris(hydroxymethyl)aminomethan (TRIS, Trometamol) ≥99,8%, weißes kristallines Pulver für die Molekularbiologie
Lieferant: MP Biomedicals
Synonyme:
Tris(hydroxymethyl)methylamin
, Tromethamin
, 2-Amino-2-(hydroxymethyl)propan-1,3-diol
, THAM
, Aminomethylidintrimethanol
, TRIS Base
, 2-Amino-2-(hydroxymethyl)-1,3-propandiol
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.
Tris may be used by itself as a buffer or as a component of mixed buffer formulations e.g. reaction buffers for restriction enzymes or nucleic acid modifying enzymes. Tris salts are used in protein crystallization at various pH values. The use of low-ionic strength Tris buffers in the formation of intermediate filaments of lamin from Caenorhabditis elegans has been described. Tris has been utilized in studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences. The use of Tris in capillary electrochromatography and UV analysis of tocopherols and tocotrienols has been reported.
Soluble in water (550 mg/ml), ethylene glycol (79,1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14,6 mg/ml), 95% ethanol (22 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0,5 mg/ml), olive oil (0,4 mg/ml), chloroform (0,05 mg/ml), and alkaline solution.
Standards: RNAse, DNase and protease free certified.
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Formel:
NH₂C(CH₂OH)₃ Molecular Weight: 121,14 g/mol |
MDL:
MFCD00004679 CAS-Nummer: 77-86-1 |
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