Anti-streptavidin is produced by hyperimmunising goats with pure streptavidin and selecting out high affinity, specific antibody by affinity chromatography on specially prepared agarose bound streptavidin. Anti-streptavidin and anti-streptavidin conjugates can be used to localise streptavidin or to amplify the signal produced by streptavidin conjugates.
Biotinylated anti-streptavidin has the unusual property of binding to streptavidin either through its antigen binding site or through its biotin residues. This product is produced by optimally biotin-labeling our affinity-purified anti-streptavidin. By following a fluorochrome conjugated streptavidin with biotinylated anti-streptavidin and adding a second layer of fluorochrome conjugated streptavidin, the fluorescent signal can be amplified. This product can be used in tissue staining, chromosome mapping, or in cell sorter analysis.