Acid phosphatase (APase) non-specifically catalyses the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.
- Soluble in distilled water and dilute buffer
- Enzyme Commission Number: E.C. 220.127.116.11
Acid phosphatase from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.
Unit definition: One unit will hydrolyse 1,0 µmole of o-carboxyphenyl phosphate per minute at 25 °C, pH 5,0.