Bovine deoxyribonuclease i (from Pancreas)

Lieferant: MP Biomedicals
ICNA0219006210EA 219 EUR
ICNA0219006210
Bovine deoxyribonuclease i (from Pancreas)
Enzyme
Dissolve at a concentration of 1 mg/ml. Dilute further to a concentration of 20 to 60 U/ml immediately before the assay.

  • Amount of enzyme that causes the fall of 1,0 relative viscosity unit in a solution of highly polymerised DNA in 10 minutes at 30 °C from the intial relative viscosity of 4,0.

Pancreatic deoxyribonuclease is unusually sensitive to physical denaturation by shaking. Mixing should be done by gentle inversion. Dissolve the standard in 1,0 ml of reagent grade water. Dilute further to a concentration of 20 to 60 U/ml. All dilutions are made in reagent grade water.

Activators: DNase I is activated by bivalent metals. Maximum activation is attained with Mg²⁺ plus Ca²⁺. It has been indicated that a metallosubstrate, such as Mg salt of DNA might be necessary. Inhibitors: According to Davidson, citrate completely inhibits magnesium-activated but not manganese-activated enzyme. DNase I is inhibited by chelating agents such as EDTA and sodium dodecyl sulphate. stabilisers: The most likely proteolytic contaminant of DNase I is chymotrypsin B. Price, et. al. report that DNase I can be stabilised against proteolytic digestion by 5 mM Ca²⁺. Di-isopropylfluorophosphate (DFP) may also be used to inhibit contaminating proteases.

DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulphate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
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