Pierce™ Conjugate Purification Kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in Tris-buffered saline.
- Procedure is designed for removal of HRP or AP enzymes from IgG antibodies (not for use with IgM, IgY, Fab fragments or streptavidin conjugates)
- Removing unconjugated enzyme from antibody conjugates often reduces background and improves overal performance of immunoassays
Lieferumfang: Kit includes IDA agarose column, 2 ml (1 column), polyacrylamide desalting column, 10 ml (1 column), Column Activator Buffer (25 ml), Elution Buffer (35 ml), 2× TBS pack (each makes 500 ml), PBS pack (makes 500 ml), and Activity Preservation Solution (1 ml).
