Peroxidase

Lieferant: MP Biomedicals
ICNA0219537301EA 42.3 EUR
ICNA0219537301 ICNA0219537310 ICNA0219537305
Peroxidase
Enzyme
HRP is a plant glycohemoprotein belongs to the ferroprotoporphyrin group of peroxidases. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens.

  • Enzyme Commission Number: E.C.1.11.1.7
  • Extinction Coefficient (E1%): 100 (403 nm)(Lit.)
  • Isoelectric point (pI): 7,2 (Lit.)

Unit definition: That amount of enzyme which catalyses the production of one milligram of purpurogallin in 20 seconds at 20 °C, at a pH of 6,0.

Inhibitors: HRP is reversibly inhibited by cyanide and sulfide at a concentration of 10−5 M. The following can also inhibit HRP: p-Aminobenzoic acid, azide, cyclopropanone, L-cystine, dichromate, ethylene thiourea, hydroxylamine, sulfite and vanadate and the divalent metals Cd, Co, Cu, Fe, Mn, Ni, Pb. Various substrate system use with enzyme =2,2'-Azino-bis(3-ethylbenz- thiazoline-6-sulfonic acid) (ABTS); o-Phenylenediamine (OPD); 3,3',5,5'-Tetramethylbenzidine (TMB); o-Dianisidine; 5-Aminosalicylic Acid (5AS); 3,3'-Diaminobenzidine (DAB); 3-Amino-9-Ethylcarbazole (AEC); 4-Chloro-1-naphthol (4C1N). RZ value=≥3,0.

Activity is observed with H₂O₂, MeOOH and EtOOH.

It is soluble in distilled water. Soluble in 0,1 M potassium phosphate buffer, pH 6,0 (monobasic potassium phosphate adjusted to pH 6,0 with 1,0 M potassium hydroxide)
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