Betain enhancer solution 5 M (5X) für PCR

Lieferant: VWR Chemicals

Synonyme: TMG

733-2451EA 4040 EUR
733-2451 733-2450 733-1361
Betain enhancer solution 5 M (5X) für PCR
Betain

Betain-Enhancer ist besonders wirksam, wenn er mit GC-reichen Regionen oder Templates mit einem hohen Grad an Sekundärstrukturen verwendet wird. Er hat eine absenkende Wirkung auf die Primer-Schmelztemperatur.


  • Enhances amplification on GC-rich and difficult DNA targets
  • Lowers the melting temperature


The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.

Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.


Bitte beachten For Research Use Only. Not for use in diagnostic procedures.

Formel: C₅H₁₁NO₂
Molecular Weight: 117,15 g/mol
Lagertemperatur: Gefrierschrank
MDL: MFCD00012123
CAS-Nummer: 107-43-7

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Spezifikation Testergebnisse

Functional test - 1x Betaine Enchancer Solution is tested for amplification of 613bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions Passes test
Volume check- fill volume >= 0.1ml per tube Passes test

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