VWR Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.
- Trypsin EDTA solutions exist in different buffers and concentrations
- Trypsin 0,25% - EDTA in HBSS without calcium, without magnesium, with phenol red
- Trypsin EDTA 1X in PBS without calcium, without magnesium without phenol red
- Trypsin EDTA 1X without calcium, without magnesium with phenol red
- Trypsin-EDTA 10X
After treatment, trypsin activity can be stopped by adding a serum media mixture or a trypsin inhibitor.